Age influences susceptibility of brain capillary endothelial cells to La Crosse virus infection and cell death.

ABSTRACT:

Background

A key factor in the development of viral encephalitis is a virus crossing the blood-brain barrier (BBB). We have previously shown that age-related susceptibility of mice to the La Crosse virus (LACV), the leading cause of pediatric arbovirus encephalitis in the USA, was associated with the ability of the virus to cross the BBB. LACV infection in weanling mice (aged around 3 weeks) results in vascular leakage in the olfactory bulb/tract (OB/OT) region of the brain, which is not observed in adult mice aged > 6-8 weeks. Thus, we studied age-specific differences in the response of brain capillary endothelial cells (BCECs) to LACV infection.

Methods

To examine mechanisms of LACV-induced BBB breakdown and infection of the CNS, we analyzed BCECs directly isolated from we

SUBMITTER: Basu R 

PROVIDER: S-EPMC8173794 | biostudies-literature | 2021 Jun

REPOSITORIES: biostudies-literature