To Plate or to Simply Unfreeze, That Is the Question for Optimal Plasmid Extraction.

ABSTRACT: Many molecular biology applications require fast plasmid DNA extraction, spurring multiple studies on how to speed up the process. It is regularly instructed in standard laboratory protocols to plate out frozen glycerol bacterial stocks prior to bacteria incubation in liquid media and subsequent plasmid extraction, although the rationale for this is often unexplained (other than for the isolation of single colonies). Given the commonality and importance of this laboratory operation, such a practice is time-consuming and laborious. To study the impact of this practice and the alternative direct culturing method, we investigated the association between bacterial cell mass and its potential influence on plasmid yields from the 2 methods. Our results showed no difference with preplating for 7

SUBMITTER: Thean RK 

PROVIDER: S-EPMC8174125 | biostudies-literature | 2021 Jul

REPOSITORIES: biostudies-literature