Tobramycin Adaptation Enhances Policing of Social Cheaters in Pseudomonas aeruginosa.

ABSTRACT: The Pseudomonas aeruginosa LasR-LasI (LasR-I) quorum sensing system regulates secreted proteases that can be exploited by cheaters, such as quorum sensing receptor-defective (lasR) mutants. lasR mutants emerge in populations growing on casein as a sole source of carbon and energy. These mutants are exploitative cheaters because they avoid the substantial cost of engaging in quorum sensing. Previous studies showed that quorum sensing increases resistance to some antibiotics, such as tobramycin. Here, we show that tobramycin suppressed the emergence of lasR mutants in casein-passaged populations. Several mutations accumulated in those populations, indicating evidence of antibiotic adaptation. We found that mutations in one gene, ptsP, increased antibiotic resistan

SUBMITTER: Abisado RG 

PROVIDER: S-EPMC8174604 | biostudies-literature | 2021 May

REPOSITORIES: biostudies-literature