Identification and evaluation of a panel of strong constitutive promoters in Listeria monocytogenes for improving the expression of foreign antigens.

ABSTRACT: Attenuated Listeria monocytogenes could be a potential vaccine vector for the immunotherapy of tumors or pathogens. However, the lack of reliable promoters has limited its ability to express foreign antigens. In the present study, 21 promoters were identified from Listeria monocytogenes through RNA-seq analysis under two pH conditions of pH 7.4 and pH 5.5. Based on the constructed fluorescence report system, 7 constitutive promoters exhibited higher strength than P_help (1.8-fold to 5.4-fold), a previously reported strong promoter. Furthermore, the selected 5 constitutive promoters exhibited higher UreB production activity than P_help (1.1-fold to 8.3-fold). Notably, a well-characterized constitutive promoter P₁₈ was found with the highest activity of fluores

SUBMITTER: Ma J 

PROVIDER: S-EPMC8175932 | biostudies-literature | 2021 Jun

REPOSITORIES: biostudies-literature