Preliminary Characterization of Two Small Insulinase-Like Proteases in Cryptosporidium parvum.

ABSTRACT: Cryptosporidium parvum is a major cause of moderate-to-severe diarrhea in humans and animals. Its compact genome contains 22 genes encoding divergent insulinase-like proteases (INS), which are poorly characterized. In this study, two small members of this family, INS-21 encoded by cgd7_2080 and INS-23 encoded by cgd5_3400, were cloned, expressed, and characterized to understand their functions. Recombinant INS-21 and INS-23 were expressed in Escherichia coli and polyclonal antibodies against these two proteins were prepared. The cgd7_2080 gene had a high transcription level during 0-2 h of in vitro C. parvum culture, while cgd5_3400 was highly transcribed at 0-6 h. INS-21 was mostly located in the apical region of sporozoites and merozoites whereas INS-23 was found as

SUBMITTER: Xu R 

PROVIDER: S-EPMC8175991 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature