Investigation of the Molecular Mechanisms by Which Endothelin-3 Stimulates Preadipocyte Growth.

ABSTRACT: Endothelins induce many biological responses, and they are composed of three peptides: ET-1, ET-2, and ET-3. Reports have indicated that ET-1 regulates cell proliferation, adipogenesis, and other cell responses and that ET-3 stimulates the growth of gastrointestinal epithelial cells and melanocytes. However, the signalling pathways of ET3 that mediate the growth of fat cells are still unclear. Using 3T3-L1 white preadipocytes, we found that ET-3 induced increases in both cell number and BrdU incorporation. Pretreatment with an ET_AR antagonist (but not an ET_BR antagonist) blocked the ET-3-induced increases in both cell number and BrdU incorporation. Additionally, BQ610 suppressed the ET-3-induced increases in phosphorylation of AMPK, c-JUN, and STAT3 proteins, and pret

SUBMITTER: Siao AC 

PROVIDER: S-EPMC8176213 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature