Voltammetric detection of vitamin D employing Au-MoS₂ hybrid as immunosensing platform.

ABSTRACT: A voltammetric immunosensor based on molybdenum sulphide (MoS₂) and gold nanoparticles (Au NPs) for the determination of 25-hydroxy vitamin D₃ (25(OH)D₃) is reported. Anti-vit D (Ab-25(OH)D₃) was immobilized onto the cysteamine-modified MoS₂ and Au NPs which were deposited onto a fluoride tin oxide (FTO) electrode (Ab/Cys/Au/MoS₂/FTO). The MoS₂ sheets were prepared by hydrothermal method followed by an in situ growth of Au film onto the MoS₂/FTO surface. Self-assembled monolayer (SAM) of cysteamine was synthesized onto the Au/MoS₂/FTO which acts as a linker to covalently bind Ab-25(OH)D₃. The Ab-25(OH)D₃-immobilized Cys/Au/MoS₂/FTO was used to detect 25(OH)D

SUBMITTER: Kaur A 

PROVIDER: S-EPMC8176887 | biostudies-literature | 2021 Jun

REPOSITORIES: biostudies-literature