Transcription factor chromatin profiling genome-wide using uliCUT&RUN in single cells and individual blastocysts.

ABSTRACT: Determining chromatin-associated protein localization across the genome has provided insight into the functions of DNA-binding proteins and their connections to disease. However, established protocols requiring large quantities of cell or tissue samples currently limit applications for clinical and biomedical research in this field. Furthermore, most technologies have been optimized to assess abundant histone protein localization, prohibiting the investigation of nonhistone protein localization in low cell numbers. We recently described a protocol to profile chromatin-associated protein localization in as low as one cell: ultra-low-input cleavage under targets and release using nuclease (uliCUT&RUN). Optimized from chromatin immunocleavage and CUT&RUN, uliCUT&RUN is a tethered enzyme-based

SUBMITTER: Patty BJ 

PROVIDER: S-EPMC8177051 | biostudies-literature | 2021 May

REPOSITORIES: biostudies-literature