Circadian misalignment by environmental light/dark shifting causes circadian disruption in colon.

ABSTRACT:

Background

Physiological circadian rhythms (CRs) are complex processes with 24-hour oscillations that regulate diverse biological functions. Chronic weekly light/dark (LD) shifting (CR disruption; CRD) in mice results in colonic hyperpermeability. However, the mechanisms behind this phenomenon are incompletely understood. One potential innovative in vitro method to study colonic CRs are colon organoids. The goals of this study were to utilize circadian clock gene Per2 luciferase reporter (Per2::Luc) mice to measure the effects of chronic LD shifting on colonic tissue circadian rhythmicity ex vivo and to determine if organoids made from shifted mice colons recapitulate the in vivo phenotype.

Methods

Non-shifted (NS) and shifted (S) BL6 Per2::Luc mice were compared after a 22-

SUBMITTER: Tran L 

PROVIDER: S-EPMC8177509 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature