Evaluation of the available cholesterol concentration in the inner leaflet of the plasma membrane of mammalian cells.

ABSTRACT: Cholesterol is an essential component of the mammalian plasma membrane involved in diverse cellular processes. Our recent quantitative imaging analysis using ratiometric cholesterol sensors showed that the available cholesterol concentration in the inner leaflet of the plasma membrane (IPM) is low in unstimulated cells and increased in a stimulus-specific manner to trigger cell signaling events. However, the transbilayer distribution of cholesterol in the plasma membrane of mammalian cells remains controversial. Here we report a systematic and rigorous evaluation of basal IPM cholesterol levels in a wide range of mammalian cells with different properties employing cholesterol sensors derived from the D4 domain of the Perfringolysin O toxin and a sterol-transfer protein, Osh4. Results consi

SUBMITTER: Buwaneka P 

PROVIDER: S-EPMC8178126 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature