Reduction in hypoxia-reoxygenation-induced myocardial mitochondrial damage with exogenous methane.

ABSTRACT: Albeit previous experiments suggest potential anti-inflammatory effect of exogenous methane (CH₄ ) in various organs, the mechanism of its bioactivity is not entirely understood. We aimed to investigate the potential mitochondrial effects and the underlying mechanisms of CH₄ in rat cardiomyocytes and mitochondria under simulated ischaemia/reperfusion (sI/R) conditions. Three-day-old cultured cardiomyocytes were treated with 2.2% CH₄ -artificial air mixture during 2-hour-long reoxygenation following 4-hour-long anoxia (sI/R and sI/R + CH₄ , n = 6-6), with normoxic groups serving as controls (SH and SH + CH₄ ; n = 6-6). Mitochondrial functions were investigated with high-resolution respirometry, and mitochondrial membrane injury was dete

SUBMITTER: Jasz DK 

PROVIDER: S-EPMC8178286 | biostudies-literature | 2021 Jun

REPOSITORIES: biostudies-literature