H₂S protects hippocampal neurons against hypoxia-reoxygenation injury by promoting RhoA phosphorylation at Ser188.

ABSTRACT: Inhibition of RhoA-ROCK pathway is involved in the H₂S-induced cerebral vasodilatation and H₂S-mediated protection on endothelial cells against oxygen-glucose deprivation/reoxygenation injury. However, the inhibitory mechanism of H₂S on RhoA-ROCK pathway is still unclear. The aim of this study was to investigate the target and mechanism of H₂S in inhibition of RhoA/ROCK. GST-RhoA^wild and GST-RhoA^S188A proteins were constructed and expressed, and were used for phosphorylation assay in vitro. Recombinant RhoA^wild-pEGFP-N1 and RhoA^S188A-pEGFP-N1 plasmids were constructed and transfected into primary hippocampal nerve cells (HNCs) to evaluate the neuroprotective mechanism of endothelial H₂S by us

SUBMITTER: Chen Y 

PROVIDER: S-EPMC8178328 | biostudies-literature | 2021 Jun

REPOSITORIES: biostudies-literature