Second harmonic generation light quantifies the ratio of type III to total (I + III) collagen in a bundle of collagen fiber.

ABSTRACT: The ratio of type III to type I collagen is important for properly maintaining functions of organs and cells. We propose a method to quantify the ratio of type III to total (type I + III) collagen (λ_III) in a given collagen fiber bundle using second harmonic generation (SHG) light. First, the relationship between SHG light intensity and the λ_III of collagen gels was examined, and the slope (k₁) and SHG light intensity at 0% type III collagen (k₂) were determined. Second, the SHG light intensity of a 100% type I collagen fiber bundle and its diameter (D) were measured, and the slope (k₃) of the relationship was determined. The λ_III in a collagen fiber bundle was estimated from these constants (k_1-3) and SHG light i

SUBMITTER: Sugita S 

PROVIDER: S-EPMC8178339 | biostudies-literature | 2021 Jun

REPOSITORIES: biostudies-literature