AAV8 transduction capacity is reduced by prior exposure to endosome-like pH conditions.

ABSTRACT: Adeno-associated virus (AAV) is an essential instrument in the neuroscientist's toolkit, which allows delivery of DNA to provide labeling with fluorescent proteins or genetic instructions to regulate gene expression. In the field of neural regeneration, the transduction of neurons enables the observation and regulation of axon growth and regeneration, and in the future will likely be a mechanism for delivering molecular therapies to promote sprouting and regeneration after central nervous system injury. Traditional formulations of AAV preparations permit efficient viral transduction under physiologic conditions, but an improved understanding of the mechanistic limitations of AAV transduction may facilitate production of more resilient AAV strains for investigative and therapeutic purposes.

SUBMITTER: Lowell JA 

PROVIDER: S-EPMC8178773 | biostudies-literature | 2021 May

REPOSITORIES: biostudies-literature