Binding site exchange kinetics revealed through efficient spin-spin dephasing of hyperpolarized ¹²⁹Xe.

ABSTRACT: Spin exchange between different chemical environments is an important observable for characterizing chemical exchange kinetics in various contexts, including protein folding, chelation chemistry, and host-guest interactions. Such spins experience effective spin-spin relaxation rate, R _2,eff, that typically shows a dispersive behavior which requires detailed analysis. Here, we describe a class of highly simplified R _2,eff behavior by relying on hyperpolarized ¹²⁹Xe as a freely exchanging ligand reporter. It provides large chemical shift separations that yield reduced expressions of both the Swift-Connick and the Carver-Richards treatment of exchange-induced relaxation. Despite observing a diamagnetic system, R _2,eff is dominat

SUBMITTER: Kunth M 

PROVIDER: S-EPMC8178811 | biostudies-literature | 2020 Oct

REPOSITORIES: biostudies-literature