In-depth evaluation of root infection systems using the vascular fungus Verticillium longisporum as soil-borne model pathogen.

ABSTRACT:

Background

While leaves are far more accessible for analysing plant defences, roots are hidden in the soil, leading to difficulties in studying soil-borne interactions. Inoculation strategies for infecting model plants with model root pathogens are described in the literature, but it remains demanding to obtain a methodological overview. To address this challenge, this study uses the model root pathogen Verticillium longisporum on Arabidopsis thaliana host plants and provides recommendations for selecting appropriate infection systems to investigate how plants cope with root pathogens.

Results

A novel root infection system is introduced, while two existing ones are precisely described and optimized. Step-by-step protocols are presented and accompanied by pathogenicity tests,

SUBMITTER: Froschel C 

PROVIDER: S-EPMC8178838 | biostudies-literature | 2021 Jun

REPOSITORIES: biostudies-literature