Reproducible and accessible analysis of transposon insertion sequencing in Galaxy for qualitative essentiality analyses.

ABSTRACT:

Background

Significant progress has been made in advancing and standardizing tools for human genomic and biomedical research. Yet, the field of next-generation sequencing (NGS) analysis for microorganisms (including multiple pathogens) remains fragmented, lacks accessible and reusable tools, is hindered by local computational resource limitations, and does not offer widely accepted standards. One such "problem areas" is the analysis of Transposon Insertion Sequencing (TIS) data. TIS allows probing of almost the entire genome of a microorganism by introducing random insertions of transposon-derived constructs. The impact of the insertions on the survival and growth under specific conditions provides precise information about genes affecting specific phenotypic characteristics. A wid

SUBMITTER: Lariviere D 

PROVIDER: S-EPMC8178898 | biostudies-literature | 2021 Jun

REPOSITORIES: biostudies-literature