A dual-fluorophore sensor approach for ratiometric fluorescence imaging of potassium in living cells.

ABSTRACT: Potassium is the most abundant intracellular metal in the body, playing vital roles in regulating intracellular fluid volume, nutrient transport, and cell-to-cell communication through nerve and muscle contraction. On the other hand, aberrant alterations in K⁺ homeostasis contribute to a diverse array of diseases spanning cardiovascular and neurological disorders to diabetes to kidney disease to cancer. There is an unmet need for studies of K⁺ physiology and pathology owing to the large differences in intracellular versus extracellular K⁺ concentrations ([K⁺]_intra = 150 mM, [K⁺]_extra = 3-5 mM). With a relative dearth of methods to reliably measure dynamic changes in intracellular K⁺ in biological

SUBMITTER: Wang Z 

PROVIDER: S-EPMC8179100 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature