Elementary processes of DNA surface hybridization resolved by single-molecule kinetics: implication for macroscopic device performance.

ABSTRACT: Direct monitoring of single-molecule reactions has recently become a promising means of mechanistic investigation. However, the resolution of reaction pathways from single-molecule experiments remains elusive, primarily because of interference from extraneous processes such as bulk diffusion. Herein, we report a single-molecule kinetic investigation of DNA hybridization on a metal surface, as an example of a bimolecular association reaction. The tip of the scanning tunneling microscope (STM) was functionalized with single-stranded DNA (ssDNA), and hybridization with its complementary strand on an Au(111) surface was detected by the increase in the electrical conductance associated with the electron transport through the resulting DNA duplex. Kinetic analyses of the conductance changes succ

SUBMITTER: Harashima T 

PROVIDER: S-EPMC8179252 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature