Monitoring the heterogeneity in single cell responses to drugs using electrochemical impedance and electrochemical noise.

ABSTRACT: Impedance spectroscopy is a widely used technique for monitoring cell-surface interactions and morphological changes, typically based on averaged signals from thousands of cells. However, acquiring impedance data at the single cell level, can potentially reveal cell-to-cell heterogeneity for example in response to chemotherapeutic agents such as doxorubicin. Here, we present a generic platform where light is used to define and localize the electroactive area, thus enabling the impedance measurements for selected single cells. We firstly tested the platform to assess phenotypic changes in breast cancer cells, at the single cell level, using the change in the cell impedance. We next show that changes in electrochemical noise reflects instantaneous responses of the cells to drugs, prior to an

SUBMITTER: Yang Y 

PROVIDER: S-EPMC8179273 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature