Precise sequencing of single protected-DNA fragment molecules for profiling of protein distribution and assembly on DNA.

ABSTRACT: Multiple DNA-interacting protein molecules are often dynamically distributed and/or assembled along a DNA molecule to adapt to their intricate functions temporally. However, analytical technology for measuring such binding behaviours is still missing. Here, we demonstrate the unique capacity of a supernuclease for a highly efficient cutting of the unprotected-DNA segments and with complete preservation of the protein-occluded DNA segments at near single-nucleotide resolution. By exploring this high-resolution cutting, an unprecedented assay that allows a precise sequencing of single protected-DNA fragment molecules (SPDFMS) was developed. As relevant applications, relevant information was gained on the respective distribution/assembly patterns and coordinated displacement of single-strande

SUBMITTER: Yuan Z 

PROVIDER: S-EPMC8179319 | biostudies-literature | 2021 Jan

REPOSITORIES: biostudies-literature