Unraveling the surface glycoprotein interaction network by integrating chemical crosslinking with MS-based proteomics.

ABSTRACT: The cell plasma membrane provides a highly interactive platform for the information transfer between the inside and outside of cells. The surface glycoprotein interaction network is extremely important in many extracellular events, and aberrant protein interactions are closely correlated with various diseases including cancer. Comprehensive analysis of cell surface protein interactions will deepen our understanding of the collaborations among surface proteins to regulate cellular activity. In this work, we developed a method integrating chemical crosslinking, an enzymatic reaction, and MS-based proteomics to systematically characterize proteins interacting with surface glycoproteins, and then constructed the surfaceome interaction network. Glycans covalently bound to proteins were employed

SUBMITTER: Sun F 

PROVIDER: S-EPMC8179341 | biostudies-literature | 2021 Jan

REPOSITORIES: biostudies-literature