The video-rate imaging of sub-10 nm plasmonic nanoparticles in a cellular medium free of background scattering.

ABSTRACT: Plasmonic nanoparticles (e.g., gold, silver) have attracted much attention for biological sensing and imaging as promising nanoprobes. Practical biomedical applications demand small gold nanoparticles (Au NPs) with a comparable size to quantum dots and fluorescent proteins. Very small nanoparticles with a size below the Rayleigh limit (usually <30-40 nm) are hard to see by light scattering using a dark-field microscope, especially within a cellular medium. A photothermal microscope is able to detect very small nanoparticles, down to a few nanometers, but the imaging speed is usually too slow (minutes to hours) to image living cell processes. Here an absorption modulated scattering microscopy (AMSM) method is presented, which allows for the imaging of sub-10 nm Au NPs within a cellul

SUBMITTER: Gao H 

PROVIDER: S-EPMC8179381 | biostudies-literature | 2021 Jan

REPOSITORIES: biostudies-literature