Visually precise, low-damage, single-cell spatial manipulation with single-pixel resolution.

ABSTRACT: The analysis of single living cells, including intracellular delivery and extraction, is essential for monitoring their dynamic biochemical processes and exploring intracellular heterogeneity. However, owing to the 2D view in bright-field microscopy and optical distortions caused by the cell shape and the variation in the refractive index both inside and around the cells, achieving spatially undistorted imaging for high-precision manipulation within a cell is challenging. Here, an accurate and visual system is developed for single-cell spatial manipulation by correcting the aberration for simultaneous bright-field triple-view imaging. Stereo information from the triple view enables higher spatial resolution that facilitates the precise manipulation of single cells. In the bright field, we

SUBMITTER: Zhang Q 

PROVIDER: S-EPMC8179525 | biostudies-literature | 2021 Feb

REPOSITORIES: biostudies-literature