CRISPR technology incorporating amplification strategies: molecular assays for nucleic acids, proteins, and small molecules.

ABSTRACT: Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) and CRISPR-associated (Cas) protein systems have transformed the field of genome editing and transcriptional modulation. Progress in CRISPR-Cas technology has also advanced molecular detection of diverse targets, ranging from nucleic acids to proteins. Incorporating CRISPR-Cas systems with various nucleic acid amplification strategies enables the generation of amplified detection signals, enrichment of low-abundance molecular targets, improvements in analytical specificity and sensitivity, and development of point-of-care (POC) diagnostic techniques. These systems take advantage of various Cas proteins for their particular features, including RNA-guided endonuclease activity, sequence-specific recognition, multiple turnover

SUBMITTER: Feng W 

PROVIDER: S-EPMC8179559 | biostudies-literature | 2021 Mar

REPOSITORIES: biostudies-literature