Multiplexed optical barcoding of cells via photochemical programming of bioorthogonal host-guest recognition.

ABSTRACT: Modern chemical and biological studies are undergoing a paradigm shift, where understanding the fate of individual cells, in an apparently homogeneous population, is becoming increasingly important. This has inculcated a growing demand for developing strategies that label individual cells with unique fluorescent signatures or barcodes so that their spatiotemporal trajectories can be mapped in real time. Among various approaches, light-regulated methods employing photocaged fluorophores have received particular attention, owing to their fine spatiotemporal control over labelling. However, their multiplexed use to barcode large numbers of cells for interrogating cellular libraries or complex tissues remains inherently challenging, due to the lack of multiple spectrally distinct photoactivate

SUBMITTER: Som A 

PROVIDER: S-EPMC8179588 | biostudies-literature | 2021 Feb

REPOSITORIES: biostudies-literature