Engineering reversible cell-cell interactions using enzymatically lipidated chemically self-assembled nanorings.

ABSTRACT: Multicellular biology is dependent on the control of cell-cell interactions. These concepts have begun to be exploited for engineering of cell-based therapies. Herein, we detail the use of a multivalent lipidated scaffold for the rapid and reversible manipulation of cell-cell interactions. Chemically self-assembled nanorings (CSANs) are formed via the oligomerization of bivalent dihydrofolate reductase (DHFR²) fusion proteins using a chemical dimerizer, bis-methotrexate. With targeting proteins fused onto the DHFR² monomers, the CSANs can target specific cellular antigens. Here, anti-EGFR or anti-EpCAM fibronectin-DHFR² monomers incorporating a CAAX-box sequence were enzymatically prenylated, then assembled into the corresponding CSANs. Both farnesyl

SUBMITTER: Wang Y 

PROVIDER: S-EPMC8179657 | biostudies-literature | 2020 Oct

REPOSITORIES: biostudies-literature