SARS-CoV-2 complete genome sequencing from the Italian Campania region using a highly automated next generation sequencing system.

ABSTRACT:

Background

Since the first complete genome sequencing of SARS-CoV-2 in December 2019, more than 550,000 genomes have been submitted into the GISAID database. Sequencing of the SARS-CoV-2 genome might allow identification of variants with increased contagiousness, different clinical patterns and/or different response to vaccines. A highly automated next generation sequencing (NGS)-based method might facilitate an active genomic surveillance of the virus.

Methods

RNA was extracted from 27 nasopharyngeal swabs obtained from citizens of the Italian Campania region in March-April 2020 who tested positive for SARS-CoV-2. Following viral RNA quantification, sequencing was performed using the Ion AmpliSeq SARS-CoV-2 Research Panel on the Genexus Integrated Sequencer, an automated te

SUBMITTER: Rachiglio AM 

PROVIDER: S-EPMC8179693 | biostudies-literature | 2021 Jun

REPOSITORIES: biostudies-literature