Characterisation of secretome-based immune responses of human leukocytes infected with various Mycobacterium tuberculosis lineages.

ABSTRACT:

Background

Differences in immune responses against different lineages of Mycobacterium tuberculosis (Mtb), and by different types of immune cell, are still poorly understood. We aimed to compare the secretome-based immune responses among three Mtb lineages and among immune-cell types. The immune responses were also investigated during infection and when the bacilli had been eliminated from the immune cells.

Methods

Human primary leukocytes were infected with strains representing three lineages of Mtb (East-Asian, Indo-Oceanic and Euro-American). Label-free GeLC MS/MS proteomic analysis of secretomes was performed. The response of each immune-cell type was compared with the appropriate interactome database for each.

Results

The expression pa

SUBMITTER: Kaewseekhao B 

PROVIDER: S-EPMC8180191 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature