NDRG1 regulates osteosarcoma cells via mediating the mitochondrial function and CSCs differentiation.

ABSTRACT:

Background

Cancer stem cells (CSCs) are mainly contributed to malignancy metastatic potential and resistant therapy of osteosarcoma (OS). The mitochondria-related apoptosis was generally accepted as the target of tumor therapy. However, the effect of N-myc downstream-regulated gene 1 (NDRG1) on CSCs and mitochondrial health in OS is still unknown.

Methods

In OS cells, MG63 and U2OS, the siRNA of NDRG1 were conducted. Transwell, western blot, RT-qPCR, and mitochondria isolation were used to identify the effect of NDRG on OS cells and mitochondria. Moreover, the differentiation-related factors of CSCs were determined.

Results

After downregulation of NDRG1, the cell viability, invasion ability decreased whereas cell apoptosis increased. The expressions profiles of fibron

SUBMITTER: Zhao T 

PROVIDER: S-EPMC8182938 | biostudies-literature | 2021 Jun

REPOSITORIES: biostudies-literature