Uncovering the chemistry of C-C bond formation in C-nucleoside biosynthesis: crystal structure of a C-glycoside synthase/PRPP complex.
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ABSTRACT:
SUBMITTER: Gao S
PROVIDER: S-EPMC8183095 | biostudies-literature | 2020 Jul
REPOSITORIES: biostudies-literature
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Uncovering the chemistry of C-C bond formation in C-nucleoside biosynthesis: crystal structure of a C-glycoside synthase/PRPP complex.
Gao Sisi S Radadiya Ashish A Li Wenbo W Liu Huanting H Zhu Wen W de Crécy-Lagard Valérie V Richards Nigel G J NGJ Naismith James H JH
Chemical communications (Cambridge, England) 20200609 55
The enzyme ForT catalyzes C-C bond formation between 5'-phosphoribosyl-1'-pyrophosphate (PRPP) and 4-amino-1H-pyrazole-3,5-dicarboxylate to make a key intermediate in the biosynthesis of formycin A 5'-phosphate by Streptomyces kaniharaensis. We report the 2.5 Å resolution structure of the ForT/PRPP complex and locate active site residues critical for PRPP recognition and catalysis. ...[more]
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