Stereochemical Trajectories of a Two-Component Regulatory System PmrA/B in a Colistin-Resistant Acinetobacter baumannii Clinical Isolate.

ABSTRACT:

Background

There is limited information on the three-dimensional (3D) prediction and modeling of the colistin resistance-associated proteins PmrA/B TCS in Acinetobacter baumannii. We aimed to evaluate the stereochemical structure and domain characterization of phosphotransferase membrane receptor A/B (PmrA/B) in an A. baumannii isolate resistant to high-level colistin, using bioinformatics tools.

Methods

The species of the isolate and its susceptibility to colistin were confirmed by PCR-sequencing and minimum inhibitory concentration assay, respectively. For 3D prediction of the PmrA/B, we used 16 template models with the highest quality (e-value <1 × 10−50).

Results

Prediction of the PmrA structure revealed a monomeric non-redundant protein consisting of 28 α-helices

SUBMITTER: Kandehkar Ghahraman MR 

PROVIDER: S-EPMC8183390 | biostudies-literature | 2021 May

REPOSITORIES: biostudies-literature