Comparative analysis of three studies measuring fluorescence from engineered bacterial genetic constructs.

ABSTRACT: Reproducibility is a key challenge of synthetic biology, but the foundation of reproducibility is only as solid as the reference materials it is built upon. Here we focus on the reproducibility of fluorescence measurements from bacteria transformed with engineered genetic constructs. This comparative analysis comprises three large interlaboratory studies using flow cytometry and plate readers, identical genetic constructs, and compatible unit calibration protocols. Across all three studies, we find similarly high precision in the calibrants used for plate readers. We also find that fluorescence measurements agree closely across the flow cytometry results and two years of plate reader results, with an average standard deviation of 1.52-fold, while the third year of plate reader results are

SUBMITTER: Beal J 

PROVIDER: S-EPMC8183995 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature