Human dermal fibroblasts support the development of human primordial/primary follicles in a 3-dimensional alginate matrix culture system.

ABSTRACT:

Background

Alginate matrix 3-dimensional culture offers the opportunity for the development and maturation of human secondary follicles in vitro. However, alginate may not be the most suitable culture system for human primordial/primary follicles in vitro. Thus, the innovation of alginate matrix 3-dimensional culture systems for human primordial/primary follicles could hold promise as an ideal approach to restoring fertility.

Methods

We extracted primordial/primary follicles from ovarian tissues collected from patients with non-ovarian benign gynecological conditions. Fibroblasts were isolated from dermal tissue from 1 male patient who had undergone posthectomy. The isolated human follicles were randomly divided into 2 groups and encapsulated within fibroblast-alginat

SUBMITTER: Wu Y 

PROVIDER: S-EPMC8184424 | biostudies-literature | 2021 May

REPOSITORIES: biostudies-literature