A Colorimetric Method for Quantifying Cis and Trans Alkenes Using an Indicator Displacement Assay.

ABSTRACT: A colorimetric indicator displacement assay (IDA) amenable to high-throughput experimentation was developed to determine the percentage of cis and trans alkenes. Using 96-well plates two steps are performed: a reaction plate for dihydroxylation of the alkenes followed by an IDA screening plate consisting of an indicator and a boronic acid. The dihydroxylation generates either erythro or threo vicinal diols from cis or trans alkenes, depending upon their syn- or anti-addition mechanisms. Threo diols preferentially associate with the boronic acid due to the creation of more stable boronate esters, thus displacing the indicator to a greater extent. The generality of the protocol was demonstrated using seven sets of cis and trans alkenes. Blind mixtures of cis and trans alkenes were made, resu

SUBMITTER: Valenzuela SA 

PROVIDER: S-EPMC8184614 | biostudies-literature | 2021 Jun

REPOSITORIES: biostudies-literature