Kinome-wide analysis of the effect of statins in colorectal cancer.

ABSTRACT:

Background

Epidemiological studies and meta-analyses show an association between statin use and a reduced incidence of colorectal cancer (CRC). We have shown that statins act on CRC through bone morphogenetic protein (BMP) signalling, but the exact cellular targets and underlying mechanism of statin action remain elusive. In this study, we set out to assess the influence of statins on global cancer cell signalling by performing an array-based kinase assay using immobilised kinase substrates spanning the entire human kinome.

Methods

CRC cells with or without Lovastatin treatment were used for kinome analysis. Findings on kinome arrays were further confirmed by immunoblotting with activity-specific antibodies. Experiments in different CRC cell lines using immunoblotting, siRNA

SUBMITTER: Ouahoud S 

PROVIDER: S-EPMC8184819 | biostudies-literature | 2021 Jun

REPOSITORIES: biostudies-literature