Dataset Information

Characterisation of circulating tumour cell phenotypes identifies a partial-EMT sub-population for clinical stratification of pancreatic cancer.

ABSTRACT:

Background

Limited accessibility of the tumour precludes longitudinal characterisation for therapy guidance in pancreatic ductal adenocarcinoma (PDAC).

Methods

We utilised dielectrophoresis-field flow fractionation (DEP-FFF) to isolate circulating tumour cells (CTCs) in 272 blood draws from 74 PDAC patients (41 localised, 33 metastatic) to non-invasively monitor disease progression.

Results

Analysis using multiplex imaging flow cytometry revealed four distinct sub-populations of CTCs: epithelial (E-CTC), mesenchymal (M-CTC), partial epithelial-mesenchymal transition (pEMT-CTC) and stem cell-like (SC-CTC). Overall, CTC detection rate was 76.8% (209/272 draws) and total CTC counts did not correlate with any clinicopathological variables. However, the proportion of pEMT-CTCs (prop-pEMT) was correlated with advanced disease, worse progression-free and overall survival in all patients, and earlier recurrence after resection.

Conclusion

Our results underscore the importance of immunophenotyping and quantifying specific CTC sub-populations in PDAC.

SUBMITTER: Semaan A

PROVIDER: S-EPMC8185080 | biostudies-literature | 2021 Jun

REPOSITORIES: biostudies-literature

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Publications

Characterisation of circulating tumour cell phenotypes identifies a partial-EMT sub-population for clinical stratification of pancreatic cancer.

Semaan Alexander A Bernard Vincent V Kim Dong U DU Lee Jaewon J JJ Huang Jonathan J Kamyabi Nabiollah N Stephens Bret M BM Qiao Wei W Varadhachary Gauri R GR Katz Matthew H MH Shen Yu Y San Lucas Francis Anthony FA Gascoyne Peter P Alvarez Hector A HA Maitra Anirban A Guerrero Paola A PA

British journal of cancer 20210330 12

<h4>Background</h4>Limited accessibility of the tumour precludes longitudinal characterisation for therapy guidance in pancreatic ductal adenocarcinoma (PDAC).<h4>Methods</h4>We utilised dielectrophoresis-field flow fractionation (DEP-FFF) to isolate circulating tumour cells (CTCs) in 272 blood draws from 74 PDAC patients (41 localised, 33 metastatic) to non-invasively monitor disease progression.<h4>Results</h4>Analysis using multiplex imaging flow cytometry revealed four distinct sub-populatio ...[more]

PMID: 33785875

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Dataset Information

Characterisation of circulating tumour cell phenotypes identifies a partial-EMT sub-population for clinical stratification of pancreatic cancer.

Background

Methods

Results

Conclusion

Publications

Characterisation of circulating tumour cell phenotypes identifies a partial-EMT sub-population for clinical stratification of pancreatic cancer.

Similar Datasets

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