Arhgef2 regulates neural differentiation in the cerebral cortex through mRNA m⁶A-methylation of Npdc1 and Cend1.

ABSTRACT: N ⁶-methyladenosine (m⁶A) is emerging as a vital factor regulating neural differentiation. Here, we report that deficiency of Arhgef2, a novel cause of a neurodevelopmental disorder we identified recently, impairs neurogenesis, neurite outgrowth, and synaptic formation by regulating m⁶A methylation. Arhgef2 knockout decreases expression of Mettl14 and total m⁶A level significantly in the cerebral cortex. m⁶A sequencing reveals that loss of Arhgef2 reduces m⁶A methylation of 1,622 mRNAs, including Npdc1 and Cend1, which are both strongly associated with cell cycle exit and terminal neural differentiation. Arhgef2 deficiency decreases m⁶A methylations of the

SUBMITTER: Zhou P 

PROVIDER: S-EPMC8185223 | biostudies-literature | 2021 Jun

REPOSITORIES: biostudies-literature