Untargeted metabolomics profiling of skeletal muscle samples from malignant hyperthermia susceptible patients.

ABSTRACT:

Purpose

Malignant hyperthermia (MH) is a potentially fatal hypermetabolic condition triggered by certain anesthetics and caused by defective calcium homeostasis in skeletal muscle cells. Recent evidence has revealed impairment of various biochemical pathways in MH-susceptible patients in the absence of anesthetics. We hypothesized that clinical differences between MH-susceptible and control individuals are reflected in measurable differences in myoplasmic metabolites.

Methods

We performed metabolomic profiling of skeletal muscle samples from MH-negative (control) individuals and MH-susceptible patients undergoing muscle biopsy for diagnosis of MH susceptibility. Cellular metabolites were extracted from 33 fresh and 87 frozen human muscle samples using solid phase microextrac

SUBMITTER: Bojko B 

PROVIDER: S-EPMC8185566 | biostudies-literature | 2021 Jun

REPOSITORIES: biostudies-literature