Ontology highlight
ABSTRACT:
SUBMITTER: Dixon G
PROVIDER: S-EPMC8185639 | biostudies-literature | 2021 Apr
REPOSITORIES: biostudies-literature
Dixon Gary G Pan Heng H Yang Dapeng D Rosen Bess P BP Jashari Therande T Verma Nipun N Pulecio Julian J Caspi Inbal I Lee Kihyun K Stransky Stephanie S Glezer Abigail A Liu Chang C Rivas Marco M Kumar Ritu R Lan Yahui Y Torregroza Ingrid I He Chuan C Sidoli Simone S Evans Todd T Elemento Olivier O Huangfu Danwei D
Science (New York, N.Y.) 20210401 6538
DNA methylation is essential to mammalian development, and dysregulation can cause serious pathological conditions. Key enzymes responsible for deposition and removal of DNA methylation are known, but how they cooperate to regulate the methylation landscape remains a central question. Using a knockin DNA methylation reporter, we performed a genome-wide CRISPR-Cas9 screen in human embryonic stem cells to discover DNA methylation regulators. The top screen hit was an uncharacterized gene, <i>QSER1 ...[more]