Devazepide suppresses cell proliferation and migration, and induces apoptosis in bladder carcinoma.

ABSTRACT:

Background

This study aimed to examine the effects of devazepide on the proliferation, migration, and apoptosis of human bladder cancer (BC) 5637 cells, and its mechanism.

Methods

A cell counting kit-8 (CCK-8) for cell viability assays, a colony formation assay, and immunofluorescence were applied to detect the effects of devazepide on the proliferation of 5637 cells. Cell cycle assay, cell apoptosis assay and wound healing assay were performed to detect the effects of devazepide on the cell cycle, apoptosis, and migration of 5637 cells. The protein expression of CyclinD1, Bcl-2-associated X protein (Bax), poly ADP-ribose polymerase 1 (PARP1), and Cleaved Caspase-3 in 5637 cells was detected by a western blot assay.

Results

The proliferation of 5637 cells was signific

SUBMITTER: Zhang H 

PROVIDER: S-EPMC8185656 | biostudies-literature | 2021 May

REPOSITORIES: biostudies-literature