Eef2k is not required for fertility in male mice.

ABSTRACT:

Background

Eukaryotic elongation factor-2 kinase (Eef2k) is a protein kinase associated with the calmodulin-induced signaling pathway and an atypical alpha-kinase family member. Eef2k-mediated phosphorylation of eukaryotic translation elongation factor 2 (Eef2) can inhibit the functionality of this protein, altering protein translation. Prior work suggests Eef2k to be overexpressed in breast, pancreatic, brain, and lung cancers wherein it may control key processes associated with apoptosis, autophagy, and cell cycle progression. The functional importance of Eef2k in the testes of male mice, however, has yet to be clarified.

Methods

A CRISPR/Cas9 approach was used to generate male Eef2k-knockout mice, which were evaluated for phenotyp

SUBMITTER: Feng T 

PROVIDER: S-EPMC8185658 | biostudies-literature | 2021 May

REPOSITORIES: biostudies-literature