SOX14 hypermethylation as a tumour biomarker in cervical cancer.

ABSTRACT:

Background

The association between SOX14 and cancer has been reported. The aim of this study was to identify and validate the potential value of SOX14 methylation in the early detection of cervical cancer.

Methods

First, we extracted the data for SOX14 methylation and expression within cervical cancer from The Cancer Genome Atlas (TCGA) database and analysed them via UALCAN, Wanderer, MEXPRESS and LinkedOmics. Subsequently, according to the bioinformatics findings, primers and probes were designed for the most significantly differentiated methylation CpG site and synthesized for methylation-specific PCR (MSP) and quantitative methylation-specific PCR (QMSP) to verify SOX14 methylation in both cervical tissuses and liquid-based cell samples. Eventually, the clinical diagnosti

SUBMITTER: Zhao J 

PROVIDER: S-EPMC8185922 | biostudies-literature | 2021 Jun

REPOSITORIES: biostudies-literature