Global transcriptome landscape of the rabbit protozoan parasite Eimeria stiedae.

ABSTRACT:

Background

Coccidiosis caused by Eimeria stiedae is a widespread and economically significant disease of rabbits. The lack of studies on the life-cycle development and host interactions of E. stiedae at the molecular level has hampered our understanding of its pathogenesis.

Methods

In this study, we present a comprehensive transcriptome landscape of E. stiedae to illustrate its dynamic development from unsporulated oocysts to sporulated oocysts, merozoites, and gametocytes, and to identify genes related to parasite-host interactions during parasitism using combined PacBio single-molecule real-time and Illumina RNA sequencing followed by bioinformatics analysis and qRT-PCR validation.

Results

In total, 12,582 non-redundant full-length transcripts were generated with an

SUBMITTER: Xie Y 

PROVIDER: S-EPMC8186055 | biostudies-literature | 2021 Jun

REPOSITORIES: biostudies-literature