MiR-361-5p as a promising qRT-PCR internal control for tumor and normal breast tissues.

ABSTRACT:

Background

One of the most widely used evaluation methods in miRNA experiments is qRT-PCR. However, selecting suitable internal controls (IC) is crucial for qRT-PCR experiments. Currently, there is no consensus on the ICs for miRNA qRT-PCR experiments in breast cancer. To this end, we tried to identify the most stable (the least expression alteration) and promising miRNAs in normal and tumor breast tissues by employing TCGA miRNA-Seq data and then experimentally validated them on fresh clinical samples.

Methods

A multi-component scoring system was used which takes into account multiple expression stability criteria as well as correlation with clinical characteristics. Furthermore, we extended the scoring system for more than two biological sub-groups. TCGA BRCA samples were

SUBMITTER: Ghanbari S 

PROVIDER: S-EPMC8186776 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature