MiR-340 Promotes Retinoblastoma Cell Proliferation, Migration and Invasion Through Targeting WIF1.

ABSTRACT:

Background

MicroRNAs (miRNAs) function as important regulators of gene expression involved in tumor pathogenesis, including retinoblastoma. However, the expression profiles and potential roles in retinoblastoma are still largely unclear.

Material and methods

Differentially expressed miRNAs (DEmiRs) and genes (DEGs) in retinoblastoma were extracted from Gene Expression Omnibus (GEO) repository. Expression levels of miR-340 and WIF1 were detected in retinoblastoma tissues and cell lines by qRT-PCR. Both gain-of-function and loss-of-function experiments were performed to explore the effects of miR-340 on cell proliferation, migration and invasion. Bioinformatics analysis and luciferase reporter assay were used to explore the interaction between miR-340 and WIF1.

Results

SUBMITTER: Li K 

PROVIDER: S-EPMC8187089 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature