Targeted mutagenesis in human iPSCs using CRISPR genome-editing tools.

ABSTRACT: Mutagenesis studies have rapidly evolved in the era of CRISPR genome editing. Precise manipulation of genes in human induced pluripotent stem cells (iPSCs) allows biomedical researchers to study the physiological functions of individual genes during development. Furthermore, such genetic manipulation applied to patient-specific iPSCs allows disease modeling, drug screening and development of therapeutics. Although various genome-editing methods have been developed to introduce or remove mutations in human iPSCs, comprehensive strategic designs taking account of the potential side effects of CRISPR editing are needed. Here we present several novel and highly efficient strategies to introduce point mutations, insertions and deletions in human iPSCs, including step-by-step experimental protoc

SUBMITTER: Long Y 

PROVIDER: S-EPMC8187269 | biostudies-literature | 2021 Jul

REPOSITORIES: biostudies-literature