GrgA overexpression inhibits Chlamydia trachomatis growth through sigma⁶⁶- and sigma²⁸-dependent mechanisms.

ABSTRACT: The obligate intracellular bacterium Chlamydia trachomatis is an important human pathogen with a biphasic developmental cycle comprised of an infectious elementary body (EB) and a replicative reticulate body (RB). Whereas σ⁶⁶, the primary sigma factor, is necessary for transcription of most chlamydial genes throughout the developmental cycle, σ²⁸ is required for expression of some late genes. We previously showed that the Chlamydia-specific transcription factor GrgA physically interacts with both of these sigma factors and activates transcription from σ⁶⁶- and σ²⁸-dependent promoters in vitro. Here, we investigated the organismal functions of GrgA. We show that overexpression of GrgA slows EB-to-RB conversion, decreases RB proliferation, and redu

SUBMITTER: Wurihan W 

PROVIDER: S-EPMC8187326 | biostudies-literature | 2021 Jul

REPOSITORIES: biostudies-literature