Study of the relationship between applied transmembrane pressure and antimicrobial activity of lysozyme.

ABSTRACT: During the processing of biomolecules by ultrafiltration, the lysozyme enzyme undergoes conformational changes, which can affect its antibacterial activity. Operational conditions are considered to be one of the main parameters responsible for such changes, especially when using the same membrane and molecule. The present study demonstrates that, the same cut-off membrane (commercial data) can result in different properties of the protein after filtration, due to their different pore network. The filtration of lysozyme, regardless of the membrane, produces a decrease in the membrane hydraulic permeability (between 10 and 30%) and an increase in its selectivity in terms of observed rejection rate (30%). For the filtrated lysozyme, it appears that the HPLC retention time increases depending

SUBMITTER: Miron SM 

PROVIDER: S-EPMC8187345 | biostudies-literature | 2021 Jun

REPOSITORIES: biostudies-literature