Comparison of the sensitivity of Western blotting between PVDF and NC membranes.
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ABSTRACT:
SUBMITTER: Xiang Y
PROVIDER: S-EPMC8187435 | biostudies-literature | 2021 Jun
REPOSITORIES: biostudies-literature
Publications
Comparison of the sensitivity of Western blotting between PVDF and NC membranes.
Xiang Yufang Y Zheng Yuanyuan Y Liu Shaobo S Liu Gang G Li Zhi Z Dong Weijie W
Scientific reports 20210608 1
Western blotting (WB) is one of the most widely used techniques to identify proteins as well as post translational modifications of proteins. The selection of electroblotted membrane is one of the key factors affecting the detection sensitivity of the protein which is transferred from gel to membrane in WB. The most common used membranes are polyvinylidene fluoride (PVDF) and nitrocellulose (NC) membranes. Which membrane of these two is more suitable for WB has not been reported so far. Here, by ...[more]
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